Composite

Part:BBa_K3804008:Design

Designed by: Ichii Shirsasuna   Group: iGEM21_UTokyo   (2021-09-29)


LasI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used tctagaGAAAGAGGGGACAAactag in BBa_J61100 as RBS and deleted 6bp(taataa) which locate in the end of LasI. Thus, the length of this sequence is 792.


Source

The part consists of sigma70promoter(BBa_J23100), RBS(BBa_J61100), LasI(BBa_C0178) and double terminator(BBa_B0015)

References