Part:BBa_K3589110

Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii, Part 2 (Phytobrick)

This basic part contains the coding sequence of the laccase from the ascomycete Botrytis aclada mutant L499F (B4). This part is codon-optimized for Chlamydomonas reinhardtii. Combined with the first part of the laccase (BBa_K3589107), a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 2 and 3 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009). To detect the target protein a 3xHA-tag from the Kaiser Collection (BBa_K3002017) is recommended. We recommend using the pAR promoter (A1-A3) (BBa_K3002003).
This part was designed to allow synthesis due to a high GC-level. It is only functional in combination with the second part (BBa_K3589110), of the laccase. Visit the page of the part BBa_K3589108 to gain a deeper understanding of the functionality of the laccase

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 57
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 57
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 575
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 57
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 57
  
• 1000
  COMPATIBLE WITH RFC[1000]