Composite

Part:BBa_K3581007:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


Hagfish intermediate filaments gamma subunit with BBa_K880005 part with HlyA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

n/a


Source

Thread biopolymer filament subunit gamma from Eptatretus stoutii (Pacific hagfish).

References

1. Jing, F., A. Guerette, P., Pavesi, A., Horbelt, N., Chwee Teck, L., J. Harrington, M., & Miserez, A. (2017). Artificial hagfish protein fibers with ultra-high and tunable stiffness, 9(35), 12908–12915. The Royal Society of Chemistry.

2. England, N. (n.d.). TEV Protease | NEB. Retrieved October 13, 2020, from https://international.neb.com/products/p8112-tev-protease#Product%20Information

3. Wikipedia. (2020, October 14). TEV Protease. Wikipedia. Retrieved October 13, 2020, from https://en.wikipedia.org/wiki/TEV_protease

4. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38

5. https://parts.igem.org/Part:BBa_K880005

6. https://parts.igem.org/Part:BBa_B0015