Part:BBa_K3453005

Synthetic RBS stem-loop designed for standard toehold switches

This part is a synthetic stem-loop containing an RBS that was designed by Pardee et al. [1] to be part of a toehold switch.

Usage and Biology

This RBS stem-loop was used to drive the expression of sfGFP-LVAtag (BBa_K2675006) under the control of the T7 promoter (BBa_K2150031) in the composite part BBa_K3453105.

It was also included in the toehold switches we designed to be specific for rosewood (BBa_K3453011, BBa_K3453012, BBa_K3453013, BBa_K3453031, BBa_K3453032, BBa_K3453033, BBa_K3453051, BBa_K3453052 and BBa_K3453053) or grapevine (BBa_K3453079 and BBa_K3453080).

References

[1] Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM, O'Connor DH, Gehrke L, Collins JJ. Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell (2016) 165, 1255-1266.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]