Composite

Part:BBa_K3317033

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)


BMC shell proteins ABJKNU from S. enterica LT2

Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) with the coresponding RBS ,this sequence is contained in plasmid pMCY30 generously provided by Yung, M. C. et. al 2017 The plasmid contains the proteins pduABJKN (BBa_K3317031), pduU protein (BBa_K3317013) and its corresponding rrnB T1 terminator (BBa_K3257020)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 41
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2424
    Illegal BamHI site found at 1761
    Illegal BamHI site found at 1900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 41
    Illegal NgoMIV site found at 1388
    Illegal AgeI site found at 1655
    Illegal AgeI site found at 1691
    Illegal AgeI site found at 2566
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None