Coding
Part:BBa_K3317013
Designed by: erick hernandez Group: iGEM19_UANL (2019-10-18)
pduU protein from S. enterica LT2
Propanediol utilization protein PduU from S. enterica LT2 (NC_003197.1)from position 7 to 46 it has a linker region that contains the ribosomal restart site, this sequence is contained in plasmid pMCY30 generously provided by Dr. Mimi C. Yung. A SpeI site and a NheI site flank the sequence for excision of pduU if necessary as described by Yung, M. C et. to 2017 this piece is part of a set which forms the BMCs (BBa_K3317033)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1
Illegal PstI site found at 161 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 398
Illegal SpeI site found at 1
Illegal PstI site found at 161 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 139
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1
Illegal PstI site found at 161 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1
Illegal PstI site found at 161
Illegal AgeI site found at 281 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |