Composite

Part:BBa_K324053:Design

Designed by: Ethan Richman   Group: iGEM10_Brown   (2010-10-25)


Constitutive - RBS - LovTAP - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 202
    Illegal PstI site found at 459
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 202
    Illegal PstI site found at 459
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 202
    Illegal PstI site found at 459
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 202
    Illegal PstI site found at 459
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 596


Design Notes

This part is modified from BBa_K191003, which contained pLac. As we desired constant production of LovTAP, this promotor was replaced. See [http://2010.igem.org/Team:Brown/Project/LRC_overview here] for more information regarding the role this part plays in our circuit.


Source

BBa_K191006 BBa_K191003

References