Composite

Part:BBa_K3237021:Design

Designed by: Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-14)


His-mRFP1-Proinsulin-CPP with furin cut sites codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1829
    Illegal XbaI site found at 1817
    Illegal XbaI site found at 1859
    Illegal SpeI site found at 1853
    Illegal PstI site found at 1367
    Illegal PstI site found at 1835
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1829
    Illegal NheI site found at 1381
    Illegal SpeI site found at 1853
    Illegal PstI site found at 1367
    Illegal PstI site found at 1835
    Illegal NotI site found at 1865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1829
    Illegal BamHI site found at 1847
    Illegal XhoI site found at 656
    Illegal XhoI site found at 1197
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1829
    Illegal XbaI site found at 1817
    Illegal XbaI site found at 1859
    Illegal SpeI site found at 1853
    Illegal PstI site found at 1367
    Illegal PstI site found at 1835
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1829
    Illegal XbaI site found at 1817
    Illegal XbaI site found at 1859
    Illegal SpeI site found at 1853
    Illegal PstI site found at 1367
    Illegal PstI site found at 1835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for C.reinhardtii <i/>. our project concerns itself with expressing insulin in micro algae. therefore, this construct was made for comparative purposes.


Source

This part is based on Tsinghua2014 and the mRFp1 gene in the registry BBa_E1010. The part was synthesized by TWIST.


===References===