Composite
Part:BBa_K3237014:Design
Designed by: Aubrey Demchuk, Kristi Turton Group: iGEM19_Lethbridge (2019-10-05)
His-mRFP1 Codon Optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 649
Illegal AgeI site found at 761 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
After the N-terminal his tag we also have added a GC linker so that the his tag addition will not affect the protein structure of mRFP1.
Source
The part sequence came from the part BBa_E1010 in the iGEM registry. The part was synthesized by IDT.