Part:BBa_K3237010:Design

His-mRFP1-SCI57-CPP with furin cut sites codon optimized for C. reinhardtii

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 363
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The part was codon optimized for Chlamydomonas reinhardtii .

Source

The mRFP1 sequence is based off of part BBa_E1010 and the SCI57 sequence was found in the literature [1]. The construct itself was synthesized by TWIST.

References

1. Hua, Q., Nakagawa, S., Jia, W., Huang, K., Philips, N., Hu, S., and Wiess, M. (2008) Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapuetic implications. The Journal of Biological Chemistry. 21, 1473-14716.

2.Liang, J., and Yang, V. (2005) Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency. Communications. 335, 734-738.

3. Hay, C., and Docherty, K. (2003) Enhanced expression of a furin-cleavable proinsulin. Journal of molecular endocrinology. 3, 597-607.