Part:BBa_K3183004

AgrA2 Quorum Sensing Transcription Factor; C. difficile

Quorum sensing transcription factor from C. difficile. It is a member of the two-component detection system, adapted from C. difficile, that binds and gets phosphorylated by AgrC2 in response to interaction with the Autoinducing peptide (AIP). The released and phosphorylated AgrA2 dimerizes with itself and becomes active. Upon activation, it binds to AgrPromoter (BBa_K3183003) which results in upregulation of transcription.¹ This gene is located in C. difficile CD196 genome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Use by Team Oxford 2019

This transcription factor is part of our detection system in L. reuteri. The composite parts containing this AgrA2 protein are BBa_K3183102, and BBa_K3183300.

Reference

1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.