Regulatory
AgrProm

Part:BBa_K3183003

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


Putative Promoter Regulated by AgrA2 in C. difficile

Putative AgrA2 Promoter for binding of AgrA2 transcription factor from AgrAC2 two-component quorum sensing system from C. difficile. The promoter is located in the agr operon and regulates agrACDB transcription in response to Quorum sensing in C. difficile.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This part was used in two composite parts for detecting C. difficile and lysing it in response using the CD27L endolysin: BBa_K3183104 and BBa_K3183300.

Reference

1. Martin, Melissa J., et al. “The Agr Locus Regulates Virulence and Colonization Genes in Clostridium Difficile 027.” Journal of Bacteriology, vol. 195, no. 16, Aug. 2013, pp. 3672–81. PubMed, doi:10.1128/JB.00473-13.

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Categories
Parameters
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