Composite

Part:BBa_K316024:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-23)


Chloraphenicol resistance gene with dif and 5amyE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reverse PCR' was used to amplify the whole vector, while adding the dif sites by primer extension. Pfu polymerase was used to ensure error-free replication.


Source

Biobrick parts BBa_K143070 (which contains BBa_K143001, BBa_K143012, BBa_K143021) and BBa_K143064 (which contains BBa_K143031, BBa_B0015)

References