Regulatory
Pveg

Part:BBa_K316001

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-12)

pVeg Constitutive promoter for Veg locus from B. subtilis

This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from BBa_K143053 by PCR. PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes. There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization in r.p.u. of Pveg promoter

Aims of experiment | Measuring the activity of BioBrick promoters using an in vivo reference standard.

Results | pVeg promoter in pSB1C3 vector, a high copy plasmid, has an 1.62 r.p.u value and in 3K3 vector, a low copy plasmid an 0.79 r.p.u. value. These values were derived by dividing signal from the production of HMS by the pVeg promoter population of cells by signal from the standard promoter J23101 (r.p.u value of 1).

See Experience page for more information


For more information about this part of our project please visit our [http://2010.igem.org/Team:Imperial_College_London/Strategy wiki] or [http://2010.igem.org/Team:Imperial_College_London/Tour/Page_One take the tour] to learn more about the project.

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Parameters
n/apVeg Constitutive promoter for Veg locus from B. subtilis