Cell

Part:BBa_K300979:Experience

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-10)

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Applications of BBa_K300979

User Reviews

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UNIPV-Pavia iGEM 2010

When cultured in YPD (30°C, 200 rpm), this yeast strain flocculated. It was impossible to measure its optical density because of the formed flocs.

In the same conditions described above, this strain took about 24 hours to grow from long term 15% glycerol stock (5 ul of the stock in 5 ml of YPD in a 15 ml tube). Before this time, the turbidity could not be appreciated.

This strain does not have the resistance to the G418 antibiotic (no colonies on YPD+G418 at 200 ug/ml plate), so the KanMX cassette (BBa_K300989) can be successfully used to select transformants or integrants.

This strain was used as a host for BBa_K300001-BBa_K300006 and BBa_K300001-BBa_K300007 integration into the genome (in the Gal system DNA) in order to test the integrative vector BBa_K300001 (see this vector's page for details).

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