Composite

Part:BBa_K300006

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-04)


mOrange with ADH1 transcriptional terminator for yeast

This part can be assembled with a yeast promoter(+RBS) upstream to drive mOrange (BBa_E2050) expression.

The BBa_K165018 part (NLS-Term with RFC23 Prefix and Suffix) has been used in this BioBrick in order to yield the transcriptional termination, while the nuclear localization signal does not work (it is after the stop codon of mOrange and the BioBrick scar TACTAGA forms another stop codon and send the translation out of frame).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None