Generator

Part:BBa_K300007:Design

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-04)

mOrange expression cassette for yeast (driven by TEF promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1172
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly.


Source

BBa_K165037 - 2010 DNA Distribution;

BBa_K300006 - UNIPV-Pavia iGEM 2010 Team.

References

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY (2004), Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. Dec;22(12):1567-72.