Part:BBa_K2924009

RBS*

Ribosome binding site* (RBS*)

Usage and Biology

The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The effectiveness of the translation depends amongst other things ¹ on the base-pairing potential between the Shine-Dalgarno sequence and the anti-Shine-Dalgarno sequence at the 3’ end of the 16S rRNA sequence and it depends on the space between the A of the core Shine-Dalgarno sequence and the first base of the start codon of the gene of interest ^{1, 2}.

RBS* was created by Heidorn (2011), is complementary to the anti-Shine-Dalgarno of Synechocystis sp. PCC 6803 and contains an optimal space of 9 base pairs ². It has been shown that RBS* is specifically in Synechocystis sp. PCC 6803 very strong ². This was tested with Escherichia coli and Synechocystis sp. PCC 6803 each containing standard biological parts BBa_B0030, BBa_B0032, BBa_B0034 and RBS*. Due to fluorescence of GFPmut3B, the transitional effectiveness could be measured and compared ².

The RBS* can therefore be used in Synechocystis for our approach to highly efficient translate target thioesterases from heterologous organism in the constructs BBa_K2924010, BBa_K2924011, BBa_K2924012, and BBa_K2924013.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]: Ringquist, S., Shinedlind, S., Barrick, D., Green, L., Binkley, J., Stormo, G. D., Gold, L. "Translation initiation in Escherichia coli: sequences within the ribosome‐binding site." Molecular microbiology 6.9 (1992): 1219-1229.

[2]: Heidorn T., Camsund, D., Huang, H.-H., Lindberg, P., Oliveira, P., Stensjö, K., Lindberg, P. "Synthetic biology in cyanobacteria: engineering and analyzing novel functions." Methods in enzymology. Vol. 497. Academic Press, 2011. 539-579.