Composite
Kai 1

Part:BBa_K2623010

Designed by: Yiming Zhu   Group: iGEM18_XMU-China   (2018-09-28)


Kai

SUMMARY

As the first part of Kai circuit, it construct the the KaiC phosphorylation oscillation circuit.
The sequence encoding for the gene is preceded by a inducable promoter BBa_R0011, an RBS BBa_B0034, BBa_K2623001, BBa_K2623002, BBa_K2623003 and followed by a double terminator BBa_B0015 in pSB1C3 for periodic oscillation of KaiC phosphorylation.
The mechanisms by which cellular oscillators keep time and transmit temporal information are poorly understood. In cyanobacteria,the timekeeping aspect of the circadian oscillator, composed of the KaiA, KaiB, and KaiC proteins, involves a cyclic progression of phosphorylation states at Ser431 and Thr432 of KaiC.

Fig.1






























Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 627
    Illegal AgeI site found at 1442
    Illegal AgeI site found at 2153
    Illegal AgeI site found at 2162
    Illegal AgeI site found at 2168
    Illegal AgeI site found at 2492
    Illegal AgeI site found at 2519
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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