Part:BBa_K2621020
CAT-Seq Barcode 4
CAT-Seq barcode collection consists of small DNA fragments with unique sequences that are used together with Nanopore Sequencing in Catalytic Activity Sequencing Pipeline. For example, if one wants to sequence two different libraries using the same Nanopore chip, it would be advised to barcode the libraries beforehand using the CAT-Seq barcodes. That is because DNA fragments from one sequencing run may present in the following one. If libraries are barcoded, these errors can be bioinformatically removed.
These barcodes can also be used in combination. For example, if a single barcode is used for each sample and there are 18 different barcodes to choose from, one can barcode 18 different samples. Yet, if used in combinations of two - up to 324 samples can potentially be barcoded using the same 18 original barcodes.
Of course, these barcodes are multi-purpose and can be used for any other required application!
CAT-Seq Barcode Inventory
Here is the list of unique CAT-Seq barcodes and their sequences:
| Biocrick number | Part name | Barcode Sequence |
|---|---|---|
| BBa_K2621017 | CAT-Seq Barcode 1 | AAGAAAGTTGTCGGTGTCTTTGTG |
| BBa_K2621018 | CAT-Seq Barcode 2 | TCGATTCCGTTTGTAGTCGTCTGT |
| BBa_K2621019 | CAT-Seq Barcode 3 | GAGTCTTGTGTCCCAGTTACCAGG |
| BBa_K2621020 | CAT-Seq Barcode 4 | TAGGGAAACACGATAGAATCCGAA |
| BBa_K2621021 | CAT-Seq Barcode 5 | CTTGTCCAGGGTTTGTGTAACCT |
| BBa_K2621022 | CAT-Seq Barcode 6 | GACTACTTTCTGCCTTTGCGAGAA |
| BBa_K2621023 | CAT-Seq Barcode 7 | GTGTTACCGTGGGAATGAATCCTT |
| BBa_K2621024 | CAT-Seq Barcode 8 | TTCAGGGAACAAACCAAGTTACGT |
| BBa_K2621025 | CAT-Seq Barcode 9 | AACTAGGCACAGCGAGTCTTGGTT |
| BBa_K2621026 | CAT-Seq Barcode 10 | AAGCGTTGAAACCTTTGTCCTCTC |
| BBa_K2621027 | CAT-Seq Barcode 11 | GTTTCATCTATCGGAGGGAATGGA |
| BBa_K2621028 | CAT-Seq Barcode 12 | CAGGTAGAAAGAAGCAGAATCGGA |
| BBa_K2621029 | CAT-Seq Barcode 13 | TCAGTGAGGATCTACTTCGACCCA |
| BBa_K2621030 | CAT-Seq Barcode 14 | TGCGTACAGCAATCAGTTACATTG |
| BBa_K2621031 | CAT-Seq Barcode 15 | CCAGTAGAAGTCCGACAACGTCAT |
| BBa_K2621032 | CAT-Seq Barcode 16 | CAGACTTGGTACGGTTGGGTAACT |
| BBa_K2621033 | CAT-Seq Barcode 17 | GGACGAAGAACTCAAGTCAAAGGC |
| BBa_K2621034 | CAT-Seq Barcode 18 | CTACTTACGAAGCTGAGGGACTGC |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |