Regulatory

Part:BBa_K259007:Design

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-09-09)


AraC Promoter fused with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107


Design Notes

The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.


Source

The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054].

The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page here.

References