Composite

Part:BBa_K2587011

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)


P_RBS_gfp_T

This construct contains a constitutive promoter (J23102: https://parts.igem.org/Part:BBa_J23102), an RBS (B0032: https://parts.igem.org/Part:BBa_B0032), a fluorescent protein: GFP (E0040m: https://parts.igem.org/Part:BBa_E0040) and a terminator (B0015: https://parts.igem.org/Part:BBa_B0015). It is built using the CIDAR MoClo toolbox for Golden Gate cloning in E.coli and can be used to track the cells due to constitutive expression of a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
Parameters
None