Project

Part:BBa_K2475003

Designed by: Shawn Cody Receno   Group: iGEM17_Waterloo   (2017-10-25)


Sup 35 Prion Domain fused to n-terminal Yellow Fluorescent Protein (PrD-nYFP)

This composite part consists of the Sup35 prion domain fused with the n-terminal fragment of the YFP complex. It does not exhibit fluorescence by itself, and must associate with the c-terminal fragment in order for fluorescence to be expressed. This was used, in conjunction with PrD-cYFP, in flow cytometry experiments to test if the aggregation domains encourage joining of the two protein halves and increases YFP expression. (Include link to other part for the registry)

Sup35 aggregation domain is from BBa_K2475000.

Used with BBa_K2475004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None