Project

Part:BBa_K2475004

Designed by: Shawn Cody Receno   Group: iGEM17_Waterloo   (2017-10-25)


Sup 35 Prion Domain fused to c-terminal Yellow Fluorescent Protein (PrD-cYFP)

This composite part consists of the Sup35 prion domain fused with the c-terminal fragment of the YFP complex. It does not exhibit fluorescence by itself, and must associate with the n-terminal fragment in order for fluorescence to be expressed. This was used, in conjunction with PrD-nYFP, in flow cytometry experiments to test if the aggregation domains encourage joining of the two protein halves and increases YFP expression.

Sup35 aggregation domain is from BBa_K2475000.

Used with BBa_K2475003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None