Part:BBa_K2446031
SV40_8_ZF54-8/43-8 promoter
This part is a mammalian synthetic promoter (SynPro) based on SV40 promoter (BBa_K2446052) and TF binding sites. We inset the TF binding sites before the promoter. minSV40 promoter is a minimized promoter from human. In this way, we can use our mammalian synthetic transcription factors (SynTFs) which is a fusing protein TF-DBD_(G4S) linker_NLS_KRAB. The TF-KRAB can bind to the TF binding sites specificallyand the KRAB domain can active the expression of minSV40 promoter [1]. It can use as a "NAND" gate regulated by SynTF-KRAB.
Figure 1: The design of SynTF-SynPros.
But for the limitation of time, we haven’t complete the test of this SynPros. Another group of SynTF-KRAB&SynPros(Sv40) works well. They are all in the table below.
| SynTFs | SynPros |
|---|---|
| Gal4-KRAB(TF-KRAB-1) BBa_K2446037 | Sv40-UAS(Sv40-UAS) BBa_K2446036 |
| ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 | SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035 |
| ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 | SV40_8_ZF_21-16 BBa_K2446030 |
| ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 | SV40_8_ZF_42-10 BBa_K2446025 |
| ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 | SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028 |
| ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 | SV40_8_ZF_54-8 BBa_K2446029 |
| ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 | SV40_4_ZFHD1 BBa_K2446032 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).
| None |