Coding

Part:BBa_K2351013

Designed by: Giel Scheepers   Group: iGEM17_Utrecht   (2017-10-23)


secreted Cas9 - PhytoBrick

Please note: PhytoBrick in Universal Acceptor Plasmid (BBa_P10500)
Prefix fusionsite: 1234 = AATG // Suffix fusionsite: 5678 = GCTT
See BBa_K2351005 for illegal BioBrick version and BBa_K2351009 for codon-corrected BioBrick version.

Improvement: This part in an improvement from BBa_K1774001 (Cas9 for expression in E. coli, by iGEM15_Hong_Kong_HKU)

Cas9 containing a signalsequence (Ig lambda-2 chain V region signal sequence) and histag (GGGHHHHHH). Codon optimized for human cell lines.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 841
    Illegal PstI site found at 2263
    Illegal PstI site found at 2467
    Illegal PstI site found at 2497
    Illegal PstI site found at 3709
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 841
    Illegal PstI site found at 2263
    Illegal PstI site found at 2467
    Illegal PstI site found at 2497
    Illegal PstI site found at 3709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 841
    Illegal PstI site found at 2263
    Illegal PstI site found at 2467
    Illegal PstI site found at 2497
    Illegal PstI site found at 3709
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 841
    Illegal PstI site found at 2263
    Illegal PstI site found at 2467
    Illegal PstI site found at 2497
    Illegal PstI site found at 3709
    Illegal NgoMIV site found at 1129
    Illegal NgoMIV site found at 2233
    Illegal NgoMIV site found at 2306
    Illegal NgoMIV site found at 2791
    Illegal NgoMIV site found at 3700
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None