Part:BBa_K2306002:Design

Secretory-abundant heat soluble protein 68234 (SAHS 68234)

This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "SAHS 68234". This is one of the several heat soluble proteins found in the tardigrades responsible for the ability of these microscopic animals to survive the harshest conditions. Upon desiccation, tardigrade proteins form a glass matrix capable of protecting other fragile biological components. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability against dehydration or even enable the possibility of stabilizing material with just a previous drying step. This BioBrick is featured in the composite part BBa_K2306007 combined with a T7 promoter BBa_R0010, a ribosome binding site BBa_k2306014 and a double terminator BBa_B0015 to control its expression. Further information about our project can be found on our [http://2017.igem.org/Team:TUDelft/Results#TDP results page].

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 451
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was already codon-optimized for E. Coli, and we made sure that there were no forbidden restriction sites present.

Source

The sequence of this part is based on that from the supplementary material of the paper by Boothby, T. C., et al. "Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation." Molecular Cell 65(6): 975-984.e975.

References

Boothby et al., 2017