RBS

Part:BBa_K2306014

Designed by: Guillermo Serena Ruiz   Group: iGEM17_TUDelft   (2017-10-24)


Efficient RBS from bacteriophage T7 gene 10 and Shine Dalgarno

Very strong ribosome binding site resulting from the combination of a gene 10 of bacteriophage T7 (g10-L) RBS and the Shine-Dalgarno RBS. The ribosome binding site derived from gene 10 of bacteriophage T7 (g10-L) causes a pronounced stimulation of expression when placed upstream of a variety of genes. However, if such a g10-L sequence is placed upstream of another RBS, as that of the Shine-Dalgarno sequence, a significant increase in the translation of the genes is observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rbs/prokaryote/constitutive
Parameters
None