Composite

Part:BBa_K200029

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)

pLacI+RBS+RcsB+TT

This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.

The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream expression of RcsB.


Usage and Biology

This composite part, comprising of subparts BBa_R0011, BBa_B0034, BBa_K200000 and BBa_B0015, was used in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project by Imperial iGEM 2009.


Characterization

Select the 'Experience' tab to see the characterization of this part by the UW-Madison 2010 Team.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None