Composite

Part:BBa_K1935016:Design

Designed by: Jean DESCARPENTRIE   Group: iGEM16_Bordeaux   (2016-10-13)


White Collar 2 under the control of pU6 promoter in C.elegans (pU6::WC2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 188
    Illegal BamHI site found at 224
    Illegal BamHI site found at 998
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1414
    Illegal NgoMIV site found at 1473
    Illegal AgeI site found at 1020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 565
    Illegal BsaI.rc site found at 933
    Illegal SapI.rc site found at 815


Design Notes

The part have been ligated with the BioBrock Assembly Method.



Source

pU6 : BBa_K1935006
WC2 : BBa_K1935005

References