Regulatory

Part:BBa_K1847008:Design

Designed by: Anna Fomitcheva Khartchenko   Group: iGEM15_ETH_Zurich   (2015-08-26)


lldRO1-J23117-lldRO2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We based our design on the natural promoter Part:BBa_K822000, keeping the disposition of the operators and changing the strength of the promoter using Aderson promoters. This way we created:

Then, we decided to change the arrangement of the operators to find out how this will affect the behavior of our system. We tried to put both operators next to each other following a promoter of different strength.

Source

Synthetic synthesis

References