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Regulatory

Part:BBa_K182005:Design

Designed by: MD. RISAT UL HAQUE, Daniel J. Leirer   Group: iGEM09_Aberdeen_Scotland   (2009-08-13)

TetR regulated by CI operator (RBS+, Term-) with LVA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

2DanAberdeenigem2009fixed.png

The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into an Ampicillin / Chloramphenicol vector (pSB1AC3), creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression.

Source

BBa_K182004 was used as the upstream part, BBa_S03518 was used as the downstream part. pSB1AC3 was our recipient vector.

References