Regulatory

Part:BBa_K182004

Designed by: MD. RISAT UL HAQUE, Daniel J. Leirer   Group: iGEM09_Aberdeen_Scotland   (2009-08-13)


Lambda CI promoter (BBa_R0051) with a point mutation in -35 region.

The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. This particular promoter has got a point mutation ( C instead of T) in the -35 region of the promoter binding site.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None