Composite

Part:BBa_K1465223:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-05)


Functional carboxysome of Halothiobacillus neapolitanus with a GFP fusion under T7 control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 882
    Illegal AgeI site found at 1833
    Illegal AgeI site found at 2514
    Illegal AgeI site found at 3417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4566
    Illegal SapI site found at 274


Design Notes

  • Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-associated proteins from Halothiobacillus neapolitanus (BBa_K1465204) into the pSB1C3 backbone of the T7 promoter (BBa_I719005).
  • Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Prefix Insertion] of the shell-associated protein (BBa_K1465204) under the control of the T7 promoter (BBa_I719005) into the pSB1C3 backbone of the shell-proteins of the carboxysome from Halothiobacillus neapolitanus fused with GFP (BBa_K1465222).

Source

BBa_I719005: T7 promoter
BBa_K1465204: shell-associated proteins of the carboxysome from Halothiobacillus neapolitanus.
BBa_K1465222: shell proteins of the carboxysome from Halothiobacillus neapolitanus fused with GFP BBa_E0040.

References