Composite

Part:BBa_K1300011:Design

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-19)


cI repressible promoter + GFP + pBAD + cI repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1065
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1005
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719


Design Notes

We designed this construct to test the GFP + pBAD + cI repressor (BBa_K611014). By placing the cI repressible promoter (BBa_R0051) directly in front of this construct, we can characterize the part.

Source

Built from existing parts in the registry though BioBrick assembly. 

cI repressible promoter [1] & GFP+pBAD+cI repressor [2]

References