Generator
Part:BBa_K1073016
Designed by: iGEM Team Braunschweig 2013 Group: iGEM13_Braunschweig (2013-09-17)
Constitutively expressed transcription regulator LasR + LVA
The device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the transcription regulator LasR (BBa_C0079).This results in a constitutively high transcription level of LasR.
Usage and Biology
In combination with N-3-oxo-dodecanoyl-HSL synthesized by LasI, LasR is able to activate the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa.
LasR binds to specific sequences in the promoter region. In the presence of N-3-oxo-dodecanoyl-HSL it activates gene expression (Medina et al., 2003).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 384
Illegal AgeI site found at 581 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |