Part:BBa_K103019:Experience

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'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with:

• protein A BBa_K103003. Resulting fusion was named His_alpha_A
• protein Z_SPA-1 BBa_K103004. Resulting fusion was named His_alpha_Z

Both fusions were overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_alpha_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). The both prey protein fusions were added to culture media and used in selection experiments. Details here and here.

User Reviews

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