DNA
p15A

Part:BBa_I50032:Design

Designed by: Reshma Shetty   Group:   (2007-04-11)

p15A replication origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 811
    Illegal AgeI site found at 1134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106


Design Notes

The boundaries of this part are quite liberal to ensure that this part is a functional origin. In constructing BBa_I50032, the replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary).

Source

We constructed BBa_I50032 by PCR using pSB3K3-P1010 as a template and amplification primers I50032-f (5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGGAATAGACTGGATGGAG-3') and I50032-r (5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACACCCCTTGTATTACTG-3').

References

<biblio>

  1. Chang-J-Bacteriol-1978 pmid=149110

</biblio>