DNA
p15A

Part:BBa_I50032

Designed by: Reshma Shetty   Group:   (2007-04-11)

p15A replication origin

BBa_I50032 is a p15A origin of replication. BBa_I50032 is the replication origin found in the pSB3* series of BioBrick vectors.

Usage and Biology

  • BBa_I50032 yields vectors with an expected copy number of 10-12 per cell ([http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning]).


When comparing this p15A to another, it looks like the documented sequence is missing a G at the 860bp. Will need further looking into.
(--vinoo)

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656109 GenBank EU496097]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 811
    Illegal AgeI site found at 1134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106


[edit]
Categories
//plasmid/component/origin
Parameters
None