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Coding

Part:BBa_K771103

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-23)

ssDsbA-PDZdomain

Component of interacting proteins of Membrane Anchor/Rudder system.


Usage and Biology

12SJTU 2PDZ.gif

The PDZ domain along with its ligand(Part:BBa_K771104) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsbA, to help the Membrane Anchor localize to the membrane correctly.

Related Parts

Part:BBa_K771104, PDZ ligand

Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand

Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345


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Categories
Parameters
n/assDsbA-PDZdomain