Composite

Part:BBa_K4056009

Designed by: Konstantinos Afordakos   Group: iGEM21_IOANNINA   (2021-10-01)


Bicistronic expression of TetX2 and EreB

Operon consisting of TetX2 and EreB that confers antibiotic resistance in tetracyclines and macrolides. TetX2 is a flavin-dependent monooxygenase that uses tetracyclines a substrate and EreB is an esterase that uses macrolides as a substrate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None