Coding
Part:BBa_K4056003
Designed by: Konstantinos Afordakos Group: iGEM21_IOANNINA (2021-09-27)
HA-tag MphR
MphR is a repressor protein binding pMphR. It downregulates the transcription of 2′-phosphotransferase Mph. It contains the TetR-type HTH DNA-binding domain [1]. Upon binding of two erythromycin molecules it undergoes a conformational change and unbinds from pMphR, allowing for Mph(A) transcription . MphR can be used in systems requiring regulation of pMphR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
None |