Coding

Part:BBa_K4056003

Designed by: Konstantinos Afordakos   Group: iGEM21_IOANNINA   (2021-09-27)


HA-tag MphR

MphR is a repressor protein binding pMphR. It downregulates the transcription of 2′-phosphotransferase Mph. It contains the TetR-type HTH DNA-binding domain [1]. Upon binding of two erythromycin molecules it undergoes a conformational change and unbinds from pMphR, allowing for Mph(A) transcription . MphR can be used in systems requiring regulation of pMphR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
Parameters
None