DNA

Part:BBa_K3866019

Designed by: Ioanna Gkoni   Group: iGEM21_Thessaly   (2021-09-23)


Stuffer PDGB A2

Fig.2:An alpha2 vector with the pdgb stuffer a2.

Usage and Biology

This translational unit is consisted of and alpha 2 vector with a stuffer. Stuffer is a part of DNA that is coding for nothing. It is used in order to swap inserts from alpha 2 or alpha2R vectors into omega vectors. This serves the GB cloning procedure



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

  • Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, et al. (2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLOS ONE 6(7): e21622. https://doi.org/10.1371/journal.pone.0021622
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