Part:BBa_K3866008

MazE antitoxin GB compatible with B3-B5

MazE antitoxin is used in the toxin-antitoxin system with mazF toxin. This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity.

Fig.2:MazE antitoxin with compatible ends for Golden Braid Cloning

Usage and Biology

The MazEF system is an extensively studied type II TA module.Toxins in type II TA systems not only inhibit DNA, RNA, or protein synthesis but are also involved in antibiotic resistance, biofilm formation, and persistent cell formation in the plasmid-based systems

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.

Fig.1:The overhangs of this part in the GoldenBraid Grammar

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 48
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

• Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107