PrRNA25- strong lactobacilli promotor
This part contains a strong constitutive promoter from Lactobacillus plantarum. A GusA activity of approximately of 1000 MU.
We confirmed the activity using mCherry as signalling protein. Simultaneously, we compared it to 7 other promotors. Five of those we added to the registry and where published by Rud et al 2006. Two were published by Team Austin, Texas, 2017. We showed that the strength exceeds most other promotors by a large margin. Even though BBa_K2253000 is described as a “strong” promotor, this novel part BBa K3855000 exceeded it 3-fold.
Experimental set up
5ml cultures of the eight different transfected Lactobacillus plantarum NC8, as well as an untransfected control, were incubated at 30°C overnight under anaerobic conditions and constant shaking. On the following day, conditions were changed to aerobic for 3-4 hours. Afterwards, the cell suspensions were diluted to different densities (OD 600 of 2, 1 and 0.1). The measurement was carried out using a Tecan reader. Samples where excited at 587 nm and emission measured at 640 nm. Sterile media was used as a reference. Experiments were carried out in triplets.
We found that the promotor expression is of very high strength. Only P1 was stronger.
Rud et al. 2006: A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum MICROBIOLOGY Volume 152, Issue 4.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC