Composite

Part:BBa_K3739042

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-09)


J23100-B0030-AKTA-his-Mfp5-B0010

It is a kind of mucin from mussels, including AKTA expression tags and 6xHis affinity tags which can let the mucin purified by tags.

Biology

Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It's side chain contains a lot of dopamine and it is the main factor of adhesion.

Usage

By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The constructed plasmid was transformed into BL21(DE3) to verify its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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