Part:BBa_K3739027

LamB1-LC1KR2-LamB2-his

We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use BBa_K3739057 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739027 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 612
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 760