Plasmid_Backbone
Part:BBa_K3271031
Designed by: Setti Belhouari Group: iGEM19_uOttawa (2019-10-21)
RFP pSB1K3 Type IIS and RFC 10 Standard Assembly Backbone
RFP is flanked by a prefix and a suffix at the 5' and 3' ends, respectively. It is also flanked by Type IIS BsaI restriction sites along with their corresponding promoter and terminator fusion sites on the 5' and 3' ends, respectively. This plasmid facilitates insertion of an entire coding cassette via either RFC 10 Standard Assembly or Type IIS Assembly
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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Categories
Parameters
None |