Part:BBa_K3271003

Gal4::EXSP pSB1K3

The prefix and suffix (EXSP) of the pSB1K3 backbone are flanked by the upstream and downstream homology sequences of the Gal4 locus from S. cerevisiae, respectively. To target a DNA part of interest to the Gal4 locus in S. cerevisiae, insert the DNA part within the prefix and the suffix using any method of your choice. Then, digest the plasmid using PmeI. This creates a DNA fragment comprised of the DNA part of interest flanked by the Gal4 locus upstream homology and the downstream homology at the 5' and 3' ends of the part, respectively. Transform the digest into S. cerevisiae using a lithium acetate protocol of choice.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 2290
  Illegal suffix found at 2312
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2290
  Illegal SpeI site found at 2313
  Illegal PstI site found at 2327
  Illegal NotI site found at 2296
  Illegal NotI site found at 2320
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2290
  Illegal XhoI site found at 1026
  Illegal XhoI site found at 2052
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2290
  Illegal suffix found at 2313
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2290
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2305
  Illegal SpeI site found at 2313
  Illegal PstI site found at 2327
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.