RBS

Part:BBa_K3257011

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-04)

T7 RBS

Efficient ribosome binding site from bacteriophage T7 gene 10.

Biology

We measured the relative fluorescence intensity of EGFP expressed by T7 promoter (BBa_K3257000) and this RBS to prove that this RBS can work.

Figure 1. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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